Skip to Main content Skip to Navigation
Journal articles

Semi-Quantitative Targeted Gas Chromatography-Mass Spectrometry Profiling Supports a Late Side-Chain Reductase Cycloartenol-to-Cholesterol Biosynthesis Pathway in Brown Algae

Abstract : Sterols are biologically important molecules that serve as membrane fluidity regulators and precursors of signaling molecules, either endogenous or involved in biotic interactions. There is currently no model of their biosynthesis pathways in brown algae. Here, we benefit from the availability of genome data and gas chromatography-mass spectrometry (GC-MS) sterol profiling using a database of internal standards to build such a model. We expand the set of identified sterols in 11 species of red, brown, and green macroalgae and integrate these new data with genomic data. Our analyses suggest that some metabolic reactions may be conserved despite the loss of canonical eukaryotic enzymes, like the sterol side-chain reductase (SSR). Our findings are consistent with the principle of metabolic pathway drift through enzymatic replacement and show that cholesterol synthesis from cycloartenol may be a widespread but variable pathway among chlorophyllian eukaryotes. Among the factors contributing to this variability, one could be the recruitment of cholesterol biosynthetic intermediates to make signaling molecules, such as the mozukulins. These compounds were found in some brown algae belonging to Ectocarpales, and we here provide a first mozukulin biosynthetic model. Our results demonstrate that integrative approaches can already be used to infer experimentally testable models, which will be useful to further investigate the biological roles of those newly identified algal pathways.
Complete list of metadata

https://hal.sorbonne-universite.fr/hal-03222505
Contributor : Gestionnaire Hal-Su Connect in order to contact the contributor
Submitted on : Monday, May 10, 2021 - 12:04:52 PM
Last modification on : Thursday, November 18, 2021 - 12:06:02 PM
Long-term archiving on: : Wednesday, August 11, 2021 - 7:17:40 PM

File

fpls-12-648426.pdf
Publication funded by an institution

Identifiers

Citation

Jean Girard, Goulven Lanneau, Ludovic Delage, Cédric Leroux, Arnaud Belcour, et al.. Semi-Quantitative Targeted Gas Chromatography-Mass Spectrometry Profiling Supports a Late Side-Chain Reductase Cycloartenol-to-Cholesterol Biosynthesis Pathway in Brown Algae. Frontiers in Plant Science, Frontiers, 2021, 12, pp.1-10. ⟨10.3389/fpls.2021.648426⟩. ⟨hal-03222505⟩

Share

Metrics

Record views

61

Files downloads

35