Imaging pathological activities of human brain tissue in organotypic culture - Sorbonne Université
Article Dans Une Revue Journal of Neuroscience Methods Année : 2018

Imaging pathological activities of human brain tissue in organotypic culture

Larissa Kraus
  • Fonction : Auteur
Jochen C Meier
  • Fonction : Auteur
Liset Menendez de la Prida
  • Fonction : Auteur

Résumé

Background-Insights into human brain diseases may emerge from tissue obtained after operations on patients. However techniques requiring transduction of transgenes carried by viral vectors cannot be applied to acute human tissue. New Method-We show that organotypic culture techniques can be used to maintain tissue from patients with three different neurological syndromes for several weeks in vitro. Optimized viral vector techniques and promoters for transgene expression are described. Results-Region-specific differences in neuronal form, firing pattern and organization as well as pathological activities were maintained over 40-50 days in culture. Both adeno-associated virus Correspondance: R. Miles
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Dates et versions

hal-03997642 , version 1 (20-02-2023)

Identifiants

Citer

Caroline Le Duigou, Etienne Savary, Mélanie Morin-Brureau, Daniel Gomez-Dominguez, André Sobczyk, et al.. Imaging pathological activities of human brain tissue in organotypic culture. Journal of Neuroscience Methods, 2018, 298, pp.33-44. ⟨10.1016/j.jneumeth.2018.02.001⟩. ⟨hal-03997642⟩
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