Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector - Sorbonne Université Access content directly
Journal Articles Zebrafish Year : 2018

Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector

Abstract

Generating chimeric zebrafish by transplantation is extremely useful for live imaging in developmental, stem cell, and cancer biology, and to answer the questions of how cells acquire, keep, and/or change their fate. However, as it is technically challenging, the use of transplantation approach remains very limited by the zebrafish community. In this study, we show that this cell grafting operation can be easily achieved by using a conventional pneumatic microinjector normally used for microinjections. Compared with previously published protocols, which need additional transplantation apparatus, this alternative transplantation method works well, but needs a simpler experimental setup, and is more accessible to all investigators.
Fichier principal
Vignette du fichier
ZEB-2017.pdf (181.84 Ko) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-04004454 , version 1 (05-03-2023)

Identifiers

Cite

Ming Shao, Xiao-Ning Cheng, Yuan-Yuan Liu, Ji-Tong Li, De-Li Shi. Transplantation of Zebrafish Cells by Conventional Pneumatic Microinjector. Zebrafish, 2018, 15 (1), pp.73-76. ⟨10.1089/zeb.2017.1495⟩. ⟨hal-04004454⟩
8 View
4 Download

Altmetric

Share

Gmail Facebook X LinkedIn More