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Journal Articles ChemBioChem Year : 2023

Analytical Tools for Dynamic Combinatorial Libraries of Cyclic Peptides

Abstract

Target‐directed dynamic combinatorial chemistry is a very attractive strategy for the discovery of bioactive peptides. However, its application has not yet been demonstrated, presumably due to analytical challenges that arise from the diversity of a peptide library with combinatorial side‐chains. We previously reported an efficient method to generate, under biocompatible conditions, large dynamic libraries of cyclic peptides grafted with amino acid’s side‐chains, by thiol‐to‐thioester exchanges. In this work, we present analytical tools to easily characterize such libraries by HPLC and mass spectrometry, and in particular to simplify the isomers’ distinction requiring sequencing by MS/MS fragmentations. After structural optimization, the cyclic scaffold exhibits a UV‐tag, absorbing at 415 nm, and an ornithine residue which favors the regioselective ring‐opening and simultaneous MS/MS fragmentation, in the gas‐phase, upon CID activation.
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Dates and versions

hal-04242176 , version 1 (14-10-2023)

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Taleen Peker, Benjamin Zagiel, Lou Rocard, Claudia Bich, Emmanuelle Sachon, et al.. Analytical Tools for Dynamic Combinatorial Libraries of Cyclic Peptides. ChemBioChem, 2023, 24 (24), pp.e202300688. ⟨10.1002/cbic.202300688⟩. ⟨hal-04242176⟩
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