A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays - Sorbonne Université Access content directly
Journal Articles Communications Biology Year : 2023

A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays

Yukiko Miyazaki
Martijn W Vos
  • Function : Author
Fiona J A Geurten
  • Function : Author
Pierre Bigeard
  • Function : Author
  • PersonId : 1376206
  • IdRef : 277164281
Hans Kroeze
  • Function : Author
Shohei Yoshioka
  • Function : Author
Mitsuhiro Arisawa
  • Function : Author
Daniel Ken Inaoka
  • Function : Author
Koen J Dechering
Blandine Franke-Fayard
  • Function : Author
Shinya Miyazaki

Abstract

Abstract Transgenic luciferase-expressing Plasmodium falciparum parasites have been widely used for the evaluation of anti-malarial compounds. Here, to screen for anti-malarial drugs effective against multiple stages of the parasite, we generate a P. falciparum reporter parasite that constitutively expresses NanoLuciferase (NanoLuc) throughout its whole life cycle. The NanoLuc-expressing P. falciparum reporter parasite shows a quantitative NanoLuc signal in the asexual blood, gametocyte, mosquito, and liver stages. We also establish assay systems to evaluate the anti-malarial activity of compounds at the asexual blood, gametocyte, and liver stages, and then determine the 50% inhibitory concentration (IC 50 ) value of several anti-malarial compounds. Through the development of this robust high-throughput screening system, we identify an anti-malarial compound that kills the asexual blood stage parasites. Our study highlights the utility of the NanoLuc reporter line, which may advance anti-malarial drug development through the improved screening of compounds targeting the human malarial parasite at multiple stages.
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Origin : Publication funded by an institution

Dates and versions

hal-04251982 , version 1 (20-10-2023)

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Yukiko Miyazaki, Martijn W Vos, Fiona J A Geurten, Pierre Bigeard, Hans Kroeze, et al.. A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays. Communications Biology, 2023, 6 (1), pp.713. ⟨10.1038/s42003-023-05078-5⟩. ⟨hal-04251982⟩
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