Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish - Sorbonne Université Access content directly
Journal Articles Nature Communications Year : 2023

Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish

Juan García-López
  • Function : Author
Alexandre Grimaldi
  • Function : Author
Zelin Chen
  • Function : Author
Claudio Meneses
Karina Bravo-Tello
  • Function : Author
Erica Bresciani
  • Function : Author
Alvaro Banderas
Shawn Burgess
Carmen Feijoo

Abstract

Abstract The current view of hematopoiesis considers leukocytes on a continuum with distinct developmental origins, and which exert non-overlapping functions. However, there is less known about the function and phenotype of ontogenetically distinct neutrophil populations. In this work, using a photoconvertible transgenic zebrafish line; Tg( mpx :Dendra2), we selectively label rostral blood island-derived and caudal hematopoietic tissue-derived neutrophils in vivo during steady state or upon injury. By comparing the migratory properties and single-cell expression profiles of both neutrophil populations at steady state we show that rostral neutrophils show higher csf3b expression and migration capacity than caudal neutrophils. Upon injury, both populations share a core transcriptional profile as well as subset-specific transcriptional signatures. Accordingly, both rostral and caudal neutrophils are recruited to the wound independently of their distance to the injury. While rostral neutrophils respond uniformly, caudal neutrophils respond heterogeneously. Collectively, our results reveal that co-existing neutrophils populations with ontogenically distinct origin display functional differences.
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Dates and versions

hal-04256141 , version 1 (24-10-2023)

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Juan García-López, Alexandre Grimaldi, Zelin Chen, Claudio Meneses, Karina Bravo-Tello, et al.. Ontogenetically distinct neutrophils differ in function and transcriptional profile in zebrafish. Nature Communications, 2023, 14 (1), pp.4942. ⟨10.1038/s41467-023-40662-7⟩. ⟨hal-04256141⟩
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