A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment - Sorbonne Université Access content directly
Journal Articles Non-Coding RNA Year : 2022

A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment

Baptiste Bogard
  • Function : Author
Gilles Tellier
  • Function : Author
Claire Francastel

Abstract

Pseudouridylation is one of the most abundant modifications found in RNAs. To identify the Pseudouridylation sites (Psi) in RNAs, several techniques have been developed, but the most common and robust is the CMC (N-cyclohexyl-N′-(2-morpholinoethyl)carbodiimide) treatment, which consists in the addition of an adduct on Psi that inhibits the reverse transcription. Here, we describe a turnkey method and a tool to design the bridging oligo (DBO), which is somewhat difficult to design. Finally, we propose a trouble-shooting guide to help users.

Dates and versions

hal-04273178 , version 1 (07-11-2023)

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Cite

Baptiste Bogard, Gilles Tellier, Claire Francastel, Florent Hubé. A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment. Non-Coding RNA, 2022, 8 (5), pp.63. ⟨10.3390/ncrna8050063⟩. ⟨hal-04273178⟩
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