Human Pancreatic β Cell lncRNAs Control Cell-Specific Regulatory Networks - Sorbonne Université Access content directly
Journal Articles Cell Metabolism Year : 2017

Human Pancreatic β Cell lncRNAs Control Cell-Specific Regulatory Networks

Leif Groop
  • Function : Author

Abstract

Recent studies have uncovered thousands of long non-coding RNAs (lncRNAs) in human pancreatic β cells. β cell lncRNAs are often cell type specific and exhibit dynamic regulation during differentiation or upon changing glucose concentrations. Although these features hint at a role of lncRNAs in β cell gene regulation and diabetes, the function of β cell lncRNAs remains largely unknown. In this study, we investigated the function of β cell-specific lncRNAs and transcription factors using transcript knockdowns and co-expression network analysis. This revealed lncRNAs that function in concert with transcription factors to regulate β cell-specific transcriptional networks. We further demonstrate that the lncRNA PLUTO affects local 3D chromatin structure and transcription of PDX1, encoding a key β cell transcription factor, and that both PLUTO and PDX1 are downregulated in islets from donors with type 2 diabetes or impaired glucose tolerance. These results implicate lncRNAs in the regulation of β cell-specific transcription factor networks.
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Dates and versions

hal-04406067 , version 1 (19-01-2024)

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Ildem Akerman, Zhidong Tu, Anthony Beucher, Delphine M.Y. Rolando, Claire Sauty-Colace, et al.. Human Pancreatic β Cell lncRNAs Control Cell-Specific Regulatory Networks. Cell Metabolism, 2017, 25 (2), pp.400-411. ⟨10.1016/j.cmet.2016.11.016⟩. ⟨hal-04406067⟩
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