New class of RNA biomarker for endometriosis diagnosis: The potential of salivary piRNA expression - Sorbonne Université Access content directly
Journal Articles European Journal of Obstetrics & Gynecology and Reproductive Biology Year : 2023

New class of RNA biomarker for endometriosis diagnosis: The potential of salivary piRNA expression

Abstract

Objectives: In contrast to miRNA expression, little attention has been given to piwiRNA (piRNA) expression among endometriosis patients. The aim of the present study was to explore the human piRNAome and to investigate a potential piRNA saliva-based diagnostic signature for endometriosis. Methods: Data from the prospective "ENDOmiRNA" study (ClinicalTrials.gov Identifier: NCT04728152) were used. Saliva samples from 200 patients were analyzed in order to evaluate human piRNA expression using the piRNA bank. Next Generation Sequencing (NGS), barcoding of unique molecular identifiers and both Artificial Intelligence (AI) and machine learning (ML) were used. For each piRNA, sensitivity, specificity, and ROC AUC values were calculated for the diagnosis of endometriosis. Results: 201 piRNAs were identified, none had an AUC ≥ 0.70, and only three piRNAs (piR-004153, piR001918, piR-020401) had an AUC between ≥ 0.6 and < 0.70. Seven were differentially expressed: piR-004153, piR-001918, piR-020401, piR-012864, piR-017716, piR-020326 and piR-016904. The respective correlation and accuracy to diagnose endometriosis according to the F1-score, sensitivity, specificity, and AUC ranged from 0 to 0.862 %, 0-0.961 %, 0.085-1, and 0.425-0.618. A correlation was observed between the patients' age (≥35 years) and piR-004153 (p = 0.002) and piR-017716 (p = 0.030). Among the 201 piRNAs, four were differentially expressed in patients with and without hormonal treatment: piR-004153 (p = 0.015), piR-020401 (p = 0.001), piR-012864 (p = 0.036) and piR-017716 (p = 0.009). Conclusion: Our results support the link between piRNAs and endometriosis physiopathology and establish its utility as a potential diagnostic biomarker using saliva samples. Per se, piRNA expression should be analyzed along with the clinical status of a patient.
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Dates and versions

hal-04444857 , version 1 (07-02-2024)

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Yohann Dabi, Stéphane Suisse, Yannick Marie, Léa Delbos, Mathieu Poilblanc, et al.. New class of RNA biomarker for endometriosis diagnosis: The potential of salivary piRNA expression. European Journal of Obstetrics & Gynecology and Reproductive Biology, 2023, 291, pp.88-95. ⟨10.1016/j.ejogrb.2023.10.015⟩. ⟨hal-04444857⟩
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