Biosensors elaborated on gold nanoparticles, a PM-IRRAS characterisation of the IgG binding efficiency. - Sorbonne Université Access content directly
Journal Articles Talanta Year : 2011

Biosensors elaborated on gold nanoparticles, a PM-IRRAS characterisation of the IgG binding efficiency.

Abstract

This work is focused on studying the grafting of gold nanoparticles (Np) on a cystamine self-assembled monolayer on gold, in order to build sensitive immunosensors. The synthesis and deposition of gold nanoparticles, 13 and 55 nm sizes, were characterised by combining Polarisation Modulation Infrared Reflection-Absorption Spectroscopy (PM-IRRAS), X-ray Photoelectron Spectroscopy (XPS) Surface Enhanced Raman Scattering (SERS), and Atomic Force Microscopy (AFM) which all indicated the formation of a dispersed layer of nanoparticles. This observation is explained by the compromise between the high reactivity of amine-terminated layers towards gold, and interparticle repulsions. Nps were then functionalised with antibody probes, and the recognition by an anti-rIgG was assayed both on planar and Np gold surfaces. The important result is that nanoparticles of 55 nm are preferable for the following reasons: they enable to build a denser and well dispersed layer and they increase both the number of receptors (IgGs) and their accessibility. Beside these geometric improvements, a net enhancement of the Raman signal was observed on the 55 nm nanoparticle layer, making this new platform promising for optical detection based biosensors.

Domains

Catalysis
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Dates and versions

hal-00668945 , version 1 (10-02-2012)

Identifiers

Cite

Anne-Laure Morel, Souhir Boujday, Christophe Méthivier, Jean-Marc Krafft, Claire-Marie Pradier. Biosensors elaborated on gold nanoparticles, a PM-IRRAS characterisation of the IgG binding efficiency.. Talanta, 2011, 85, pp.35-42. ⟨10.1016/j.talanta.2011.02.028⟩. ⟨hal-00668945⟩
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