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Journal Articles PLoS Pathogens Year : 2015

Complement-Opsonized HIV-1 Overcomes Restriction in Dendritic Cells

Abstract

DCs express intrinsic cellular defense mechanisms to specifically inhibit HIV-1 replication. Thus, DCs are productively infected only at very low levels with HIV-1, and this non-permissiveness of DCs is suggested to go along with viral evasion. We now illustrate that complement-opsonized HIV-1 (HIV-C) efficiently bypasses SAMHD1 restriction and productively infects DCs including BDCA-1 DCs. Efficient DC infection by HIV-C was also observed using single-cycle HIV-C, and correlated with a remarkable elevated SAMHD1 T592 phosphorylation but not SAMHD1 degradation. If SAMHD1 phosphorylation was blocked using a CDK2-inhibitor HIV-C-induced DC infection was also significantly abrogated. Additionally, we found a higher maturation and co-stimulatory potential, aberrant type I interferon expression and signaling as well as a stronger induction of cellular immune responses in HIV-C-treated DCs. Collectively, our data highlight a novel protective mechanism mediated by complement opsonization of HIV to effectively promote DC immune functions , which might be in the future exploited to tackle HIV infection.
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hal-01223768 , version 1 (03-11-2015)

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Wilfried Posch, Marion Steger, Ulla Knackmuss, Michael Blatzer, Hanna-Mari Baldauf, et al.. Complement-Opsonized HIV-1 Overcomes Restriction in Dendritic Cells. PLoS Pathogens, 2015, 115 (6), pp.e1005005. ⟨10.1371/journal.ppat.1005005⟩. ⟨hal-01223768⟩
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