Deep sequencing of amplified Prasinovirus and host green algal genes from an Indian Ocean transect reveals interacting trophic dependencies and new genotypes - Sorbonne Université Accéder directement au contenu
Article Dans Une Revue Environmental Microbiology Reports Année : 2015

Deep sequencing of amplified Prasinovirus and host green algal genes from an Indian Ocean transect reveals interacting trophic dependencies and new genotypes

Pascal Hingamp
Hiroyuki Ogata

Résumé

High-throughput sequencing of Prasinovirus DNA polymerase and host green algal (Mamiellophyceae) ribosomal RNA genes was used to analyse the diversity and distribution of these taxa over a ∼10 000 km latitudinal section of the Indian Ocean. New viral and host groups were identified among the different trophic conditions observed, and highlighted that although unknown prasinoviruses are diverse, the cosmopolitan algal genera Bathycoccus, Micromonas and Ostreococcus represent a large proportion of the host diversity. While Prasinovirus communities were correlated to both the geography and the environment, host communities were not, perhaps because the genetic marker used lacked sufficient resolution. Nevertheless, analysis of single environmental variables showed that eutrophic conditions strongly influence the distributions of both hosts and viruses. Moreover, these communities were not correlated, in their composition or specific richness. These observations could result from antagonistic dynamics, such as that illustrated in a prey–predator model, and/or because hosts might be under a complex set of selective pressures. Both of these reasons must be considered to interpret environmental surveys of viruses and hosts, because covariation does not always imply interaction.
Fichier principal
Vignette du fichier
Clerissi_2015_Deep_sequencing_of.pdf (419.08 Ko) Télécharger le fichier
Vignette du fichier
EMI4_12345_supp-0001-Supporting_information_figures_clerissi_et_al_EMIR.pdf (9.54 Mo) Télécharger le fichier
EMI4_12345_supp-0002-Supporting_information_methods_clerissi_et_al_EMIR.doc (143.5 Ko) Télécharger le fichier
EMI4_12345_supp-0003-Supporting_information_tables_clerissi_et_al_EMIR.doc (128 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Format : Figure, Image
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01246259 , version 1 (04-01-2016)

Identifiants

Citer

Camille Clerissi, Yves Desdevises, Sarah Romac, Stéphane Audic, Colomban de Vargas, et al.. Deep sequencing of amplified Prasinovirus and host green algal genes from an Indian Ocean transect reveals interacting trophic dependencies and new genotypes. Environmental Microbiology Reports, 2015, 7 (6), pp.979-989. ⟨10.1111/1758-2229.12345⟩. ⟨hal-01246259⟩
465 Consultations
266 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More