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bz-rates: a web-tool to estimate mutation rates from fluctuation analysis

Abstract : Fluctuation analyses is the standard experimental method for measuring mutation rates in microorganisms. The appearance of mutants is classically described by a Luria-Delbrück distribution composed of two parameters: the number of mutations per culture (m) and the differential growth rate between mutant and wild-type cells (b). A precise estimation of these 2 parameters is a prerequisite to the calculation of the mutation rate. Here, we developed bz-rates, a web-tool to calculate mutation rates that provides three useful advances over existing web-tools. First, it allows taking into account b, the differential growth rate between mutant and wild-type cells, in the estimation of m with the Generating Function (GF). Secondly, bz-rates allows the user to take into account a deviation from the Luria-Delbrück distribution called z, the plating efficiency, in the estimation of m. Finally, the web-site provides a graphical visualization of the goodness-of-fit between the experimental data and the model.
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Alexandre Gillet-Markowska, Guillaume Louvel, Gilles Fischer. bz-rates: a web-tool to estimate mutation rates from fluctuation analysis. G3, Genetics Society of America, 2015, 5 (11), pp.2323-2327. ⟨10.1534/g3.115.019836⟩. ⟨hal-01264305⟩

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