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Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress

Abstract : The Arabidopsis non-specific phospholipase C 1 (NPC) protein family is encoded by the genes NPC1 – NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.
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Zuzana Křcková, Jitka Brouzdová, Michal Daňek, Daniela Kocourková, Dominique Rainteau, et al.. Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress. Frontiers in Plant Science, Frontiers, 2015, 6, pp.928. ⟨10.3389/fpls.2015.00928⟩. ⟨hal-01266347⟩

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