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Journal Articles Biophysical Journal Year : 2014

Border Forces and Friction Control Epithelial Closure Dynamics

Philippe Marcq
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Olivier Cochet-Escartin
Jonas Ranft
Pascal Silberzan
Philippe Marcq

Abstract

We study the closure dynamics of a large number of well-controlled circular apertures within an epithelial monolayer, where the collective cell migration responsible for epithelization is triggered by the removal of a spatial constraint rather than by scratching. Based on experimental observations, we propose a physical model that takes into account border forces, friction with the substrate, and tissue rheology. Border protrusive activity drives epithelization despite the presence of a contractile actomyosin cable at the periphery of the wound. The closure dynamics is quantified by an epithelization coefficient, defined as the ratio of protrusive stress to tissue-substrate friction, that allows classification of different phenotypes. The same analysis demonstrates a distinct signature for human cells bearing the oncogenic RasV12 mutation, demonstrating the potential of the approach to quantitatively characterize metastatic transformations.

Dates and versions

hal-01319448 , version 1 (20-05-2016)

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Philippe Marcq, Olivier Cochet-Escartin, Jonas Ranft, Pascal Silberzan, Philippe Marcq. Border Forces and Friction Control Epithelial Closure Dynamics. Biophysical Journal, 2014, 106 (1), pp.65-73. ⟨10.1016/j.bpj.2013.11.015⟩. ⟨hal-01319448⟩
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