Fischer carbene mediated covalent grafting of a peptide nucleic acid on gold surfaces and IR optical detection of DNA hybridization with a transition metalcarbonyl label - Sorbonne Université Accéder directement au contenu
Article Dans Une Revue Applied Surface Science Année : 2016

Fischer carbene mediated covalent grafting of a peptide nucleic acid on gold surfaces and IR optical detection of DNA hybridization with a transition metalcarbonyl label

Résumé

Amine-reactive surfaces comprising N-hydroxysuccinimide ester groups as well as much more unusual Fischer alkoxymetallocarbene groups were generated on gold-coated surfaces via self-assembled monolayers of carboxy- and azido-terminated thiolates, respectively. These functions were further used to immobilize homothymine peptide nucleic acid (PNA) decamer in a covalent fashion involving the primary amine located at its N-terminus. These stepwise processes were monitored by polarization modulation reflection – absorption infrared spectroscopy (PM-RAIRS) that gave useful information on the molecular composition of the organic layers. PNA grafting and hybridization with complementary DNA strand were successfully transduced by quartz crystal microbalance (QCM) measurements. Unfortunately, attempts to transduce the hybridization optically by IR in a label-free fashion were inconclusive. Therefore we undertook to introduce an IR reporter group, namely a transition metalcarbonyl (TMC) entity at the 5′ terminus of complementary DNA. Evidence for the formation of PNA-DNA heteroduplex was brought by the presence of ν(Ctriple bond; length of mdashO) bands in the 2000 cm−1 region of the IR spectrum of the gold surface owing to the metalcarbonyl label.

Domaines

Chimie
Fichier principal
Vignette du fichier
Srivastava_Fischer_carbene.pdf (1.65 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01325035 , version 1 (01-06-2016)

Identifiants

Citer

Pratima Srivastava, Mahsa Ghasemi, Namrata Ray, Amitabha Sarkar, Jana Kocabova, et al.. Fischer carbene mediated covalent grafting of a peptide nucleic acid on gold surfaces and IR optical detection of DNA hybridization with a transition metalcarbonyl label. Applied Surface Science, 2016, 385, pp.47-55. ⟨10.1016/j.apsusc.2016.05.058⟩. ⟨hal-01325035⟩
430 Consultations
158 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More