Selective tools for the solid-phase extraction of Ochratoxin A from various complex samples: immunosorbents, oligosorbents, and molecularly imprinted polymers - Sorbonne Université Access content directly
Journal Articles Analytical and Bioanalytical Chemistry Year : 2016

Selective tools for the solid-phase extraction of Ochratoxin A from various complex samples: immunosorbents, oligosorbents, and molecularly imprinted polymers

Abstract

The evolution of the instrumentation in terms of separation and detection has allowed a real improvement of the sensitivity and the analysis time. However, the analysis of ultra-traces of toxins such as Ochratoxin A (OTA) from complex samples (foodstuff, biological fluids…) still requires a step of purification and of preconcentration before their chromatographic determination. In this context, extraction sorbents leading to a molecular recognition mechanism appear as powerful tools for the selective extraction of OTA and of its structural analogs in order to obtain more reliable and sensitive quantitative analyses of these compounds in complex media. Indeed, immunosorbents and oligosorbents that are based on the use of immobilized antibodies and of aptamers respectively and that are specific to OTA allow its selective clean-up from complex samples with high enrichment factors. Similar molecular recognition mechanisms can also be obtained by developing molecularly imprinted polymers whose synthesis leads to the formation of cavities that are specific to OTA thus mimicking the recognition site of the biomolecules. Therefore, the principle, the advantages, the limits of these different types of extraction tools and their complementary behaviors will be presented. The introduction of these selective tools in miniaturized devices will also be discussed.
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Dates and versions

hal-01373783 , version 1 (29-09-2016)

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Valérie Pichon, Audrey Combès. Selective tools for the solid-phase extraction of Ochratoxin A from various complex samples: immunosorbents, oligosorbents, and molecularly imprinted polymers. Analytical and Bioanalytical Chemistry, 2016, 408 (25), pp.6983-6999. ⟨10.1007/s00216-016-9886-0⟩. ⟨hal-01373783⟩
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