Dissection of quantitative traits by bulk segregant mapping in a protoploid yeast species.

Abstract : Since more than a decade ago, Saccharomyces cerevisiae has been used as a model to dissect complex traits, revealing the genetic basis of a large number of traits in fine detail. However, to have a more global view of the genetic architecture of traits across species, the examination of the molecular basis of phenotypes within non-conventional species would undoubtedly be valuable. In this respect, the Saccharomycotina yeasts represent ideal and potential non-model organisms. Here we sought to assess the feasibility of genetic mapping by bulk segregant analysis in the protoploid Lachancea kluyveri (formerly S. kluyveri) yeast species, a distantly related species to S. cerevisiae For this purpose, we designed a fluorescent mating-type marker, compatible with any mating-competent strains representative of this species, to rapidly create a large population of haploid segregants (>10(5) cells). Quantitative trait loci can be mapped by selecting and sequencing an enriched pool of progeny with extreme phenotypic values. As a test bed, we applied this strategy and mapped the causal loci underlying halotolerance phenotypes in L. kluyveri Overall, this study demonstrates that bulk segregant mapping is a powerful way for investigating the genetic basis of natural variations in non-model yeast organisms and more precisely in L. kluyveri.
Type de document :
Article dans une revue
FEMS Yeast Research, Oxford University Press (OUP), 2016, 16 (5)
Liste complète des métadonnées

https://hal.sorbonne-universite.fr/hal-01588206
Contributeur : Francesco Oteri <>
Soumis le : vendredi 15 septembre 2017 - 12:09:09
Dernière modification le : lundi 2 juillet 2018 - 15:07:03

Identifiants

  • HAL Id : hal-01588206, version 1
  • PUBMED : 27371856

Citation

Anastasie Sigwalt, Claudia Caradec, Christian Brion, Jing Hou, Jacky De Montigny, et al.. Dissection of quantitative traits by bulk segregant mapping in a protoploid yeast species.. FEMS Yeast Research, Oxford University Press (OUP), 2016, 16 (5). 〈hal-01588206〉

Partager

Métriques

Consultations de la notice

179