A novel colorimetric and fluorescent turn-on pH sensor with a notably large Stokes shift for its application
Résumé
A novel naked-eye colorimetric and fluorescent turn-on pH sensor based on naphthalenone scaffold was rationally designed and facilely synthesized. The probe exhibited strong alkaline pH-dependent (pKa=9.92) behavior, high selectivity, sensitivity, high quantum efficiency (Ф= 0.64) and rapid respond to pH fluctuations (within 1 min) in the range of 9.0-14.0. In addition, probe DDTM displayed a notably large Stokes shift of 198 nm. The response mechanism of the fluorescent probes was proposed to be caused by OH-induced structure changes from the nonfluorescent DDTM form to the highly emissive addition product (more than 150-fold fluorescence enhancement) along with ICT changes. Live cell imaging data revealed that probe DDTM could selectively monitor pH changes with low cytotoxicity and cell membrane permeability. Therefore, this probe could be acted as an effective intracellular pH imaging sensor under extreme alkaline condition in the biomedical and biological fields.
Domaines
Chimie| Origine | Fichiers produits par l'(les) auteur(s) |
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