HIV-1 Envelope Recognition by Polyreactive and Cross-Reactive Intestinal B Cells - Sorbonne Université Access content directly
Journal Articles Cell Reports Year : 2019

HIV-1 Envelope Recognition by Polyreactive and Cross-Reactive Intestinal B Cells

Timothée Bruel
Florence Guivel-Benhassine
Thierry Hieu
Olivier Schwartz
  • Function : Author
  • PersonId : 999827

Abstract

Mucosal immune responses to HIV-1 involve the recognition of the viral envelope glycoprotein (gp)160 by tissue-resident B cells and subsequent secretion of antibodies. To characterize the B cells “sensing” HIV-1 in the gut of infected individuals, we probed monoclonal antibodies produced from single intestinal B cells binding to recombinant gp140 trimers. A large fraction of mucosal B cell antibodies were polyreactive and showed only low affinity to HIV-1 envelope glycoproteins, particularly the gp41 moiety. A few high-affinity gp140 antibodies were isolated but lacked neutralizing, potent ADCC, and transcytosis-blocking capacities. Instead, they displayed cross-reactivity with defined self-antigens. Specifically, intestinal HIV-1 gp41 antibodies targeting the heptad repeat 2 region (HR2) cluster II cross-reacted with the p38α mitogen-activated protein kinase 14 (MAPK14). Hence, physiologic polyreactivity of intestinal B cells and molecular mimicry-based self-reactivity of HIV-1 antibodies are two independent phenomena, possibly diverting and/or impairing mucosal humoral immunity to HIV-1.
Fichier principal
Vignette du fichier
1-s2.0-S2211124719303481-main.pdf (6.1 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

hal-02102470 , version 1 (17-04-2019)

Licence

Attribution - NonCommercial - NoDerivatives

Identifiers

Cite

Cyril Planchais, Ayrin Kök, Alexia Kanyavuz, Valérie Lorin, Timothée Bruel, et al.. HIV-1 Envelope Recognition by Polyreactive and Cross-Reactive Intestinal B Cells. Cell Reports, 2019, 27 (2), pp.572-585.e7. ⟨10.1016/j.celrep.2019.03.032⟩. ⟨hal-02102470⟩
110 View
115 Download

Altmetric

Share

Gmail Facebook X LinkedIn More