Non-conventional Axonal Organelles Control TRPM8 Ion Channel Trafficking and Peripheral Cold Sensing - Sorbonne Université
Article Dans Une Revue Cell Reports Année : 2020

Non-conventional Axonal Organelles Control TRPM8 Ion Channel Trafficking and Peripheral Cold Sensing

Résumé

TRPM8 is the main ion channel responsible for cold transduction in the somatosensory system. Nerve terminal availability of TRPM8 determines cold sensitivity, but how axonal secretory organelles control channel delivery remains poorly understood. Here we examine the distribution of TRPM8 and trafficking organelles in cold-sensitive peripheral axons and disrupt trafficking by targeting the ARF-GEF GBF1 pharmacologically or the small GTPase RAB6 by optogenetics. In axons of the sciatic nerve, inhibition of GBF1 interrupts TRPM8 trafficking and increases association with the trans-Golgi network, LAMP1, and Golgi satellites, which distribute profusely along the axonal shaft. Accordingly, both TRPM8-dependent ongoing activity and cold-evoked responses reversibly decline upon GBF1 inhibition in nerve endings of corneal cold thermoreceptors. Inhibition of RAB6, which also associates to Golgi satellites, decreases cold-induced responses in vivo. Our results support a non-conventional axonal trafficking mechanism controlling the availability of TRPM8 in axons and cold sensitivity in the peripheral nervous system.
Fichier principal
Vignette du fichier
1-s2.0-S2211124720303247-main.pdf (3.8 Mo) Télécharger le fichier
Origine Publication financée par une institution
Loading...

Dates et versions

hal-02552332 , version 1 (23-04-2020)

Identifiants

Citer

Víctor Hugo Cornejo, Carolina González, Matías Campos, Leslie Vargas-Saturno, María de Los Ángeles Juricic, et al.. Non-conventional Axonal Organelles Control TRPM8 Ion Channel Trafficking and Peripheral Cold Sensing. Cell Reports, 2020, 30 (13), pp.4505-4517. ⟨10.1016/j.celrep.2020.03.017⟩. ⟨hal-02552332⟩
52 Consultations
132 Téléchargements

Altmetric

Partager

More