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A far-red fluorescent chemogenetic reporter for in vivo molecular imaging

Abstract : Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially nonfluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae and chicken embryo. Beyond enabling genetic encoding of far-red fluorescence, frFAST allowed the design of a farred chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.
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Submitted on : Tuesday, December 1, 2020 - 4:53:47 PM
Last modification on : Thursday, April 7, 2022 - 1:58:11 PM
Long-term archiving on: : Tuesday, March 2, 2021 - 8:02:21 PM

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Chenge Li, Alison Tebo, Marion Thauvin, Marie-Aude Plamont, Michel Volovitch, et al.. A far-red fluorescent chemogenetic reporter for in vivo molecular imaging. Angewandte Chemie International Edition, Wiley-VCH Verlag, 2020, 59 (41), pp.17917-17923. ⟨10.1002/anie.202006576⟩. ⟨hal-03034345⟩

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