A far-red fluorescent chemogenetic reporter for in vivo molecular imaging - Sorbonne Université
Journal Articles Angewandte Chemie International Edition Year : 2020

A far-red fluorescent chemogenetic reporter for in vivo molecular imaging

Abstract

Far-red emitting fluorescent labels are highly desirable for spectral multiplexing and deep tissue imaging. Here, we describe the generation of frFAST (far-red Fluorescence Activating and absorption Shifting Tag), a 14-kDa monomeric protein that forms a bright far-red fluorescent assembly with (4-hydroxy-3-methoxy-phenyl)allylidene rhodanine (HPAR-3OM). As HPAR-3OM is essentially nonfluorescent in solution and in cells, frFAST can be imaged with high contrast in presence of free HPAR-3OM, which allowed the rapid and efficient imaging of frFAST fusions in live cells, zebrafish embryo/larvae and chicken embryo. Beyond enabling genetic encoding of far-red fluorescence, frFAST allowed the design of a farred chemogenetic reporter of protein-protein interactions, demonstrating its great potential for the design of innovative far-red emitting biosensors.
Fichier principal
Vignette du fichier
Li et al. - A Far-Red Emitting Fluorescent Chemogenetic Report.pdf (6.94 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03034345 , version 1 (01-12-2020)

Identifiers

Cite

Chenge Li, Alison G Tebo, Marion Thauvin, Marie-Aude Plamont, Michel Volovitch, et al.. A far-red fluorescent chemogenetic reporter for in vivo molecular imaging. Angewandte Chemie International Edition, 2020, 59 (41), pp.17917-17923. ⟨10.1002/anie.202006576⟩. ⟨hal-03034345⟩
78 View
105 Download

Altmetric

Share

More