High-Throughput Metabolomics Using Flow Injection Analysis and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry - Sorbonne Université
Chapitre D'ouvrage Année : 2021

High-Throughput Metabolomics Using Flow Injection Analysis and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Résumé

The hyphenation of flow injection analysis (FIA) with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is an efficient approach usable to perform high throughput and very high resolution metabolomic data acquisition. Instrumental and analytical conditions for performing FIA-MS are provided. The procedure to optimize dilution factor of biological samples as well as to evaluate quality of acquisition data are also described. In this protocol, urine is chosen as a matrix example to illustrate the application of procedures. Last, some indications on an adapted data processing are given.

Domaines

Chimie
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Dates et versions

hal-03133036 , version 1 (05-02-2021)

Identifiants

  • HAL Id : hal-03133036 , version 1

Citer

Estelle Rathahao-Paris, Sandra Alves, Alain Paris. High-Throughput Metabolomics Using Flow Injection Analysis and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Metabolomics, 159, Humana, pp.9--23, 2021, Neuromethods, 978-1-07-160864-7. ⟨hal-03133036⟩
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