Skip to Main content Skip to Navigation
Journal articles

Th1-Polarized, Dengue Virus-Activated Human Mast Cells Induce Endothelial Transcriptional Activation and Permeability

Abstract : Dengue virus (DENV), an arbovirus, strongly activates mast cells (MCs), which are key immune cells for pathogen immune surveillance. In animal models, MCs promote clearance of local peripheral DENV infections but, conversely, also promote pathological vascular leakage when widely activated during systemic DENV infection. Since DENV is a human pathogen, we sought to ascertain whether a similar phenomenon could occur in humans by characterizing the products released by human MCs (huMCs) upon direct (antibody-independent) DENV exposure, using the phenotypically mature huMC line, ROSA. DENV did not productively infect huMCs but prompted huMC release of proteases and eicosanoids and induced a Th1-polarized transcriptional profile. In co-culture and trans-well systems, huMC products activated human microvascular endothelial cells, involving transcription of vasoactive mediators and increased monolayer permeability. This permeability was blocked by MC-stabilizing drugs, or limited by drugs targeting certain MC products. Thus, MC stabilizers are a viable strategy to limit MC-promoted vascular leakage during DENV infection in humans.
Document type :
Journal articles
Complete list of metadata

https://hal.sorbonne-universite.fr/hal-03152386
Contributor : Gestionnaire Hal-Su <>
Submitted on : Thursday, February 25, 2021 - 3:23:56 PM
Last modification on : Monday, March 1, 2021 - 11:34:24 AM

File

viruses-12-01379-v2.pdf
Publication funded by an institution

Identifiers

Citation

Ayesa Syenina, Wilfried Saron, Cyril Jagaraj, Siham Bibi, Michel Arock, et al.. Th1-Polarized, Dengue Virus-Activated Human Mast Cells Induce Endothelial Transcriptional Activation and Permeability. Viruses, MDPI, 2020, 12 (12), pp.1379. ⟨10.3390/v12121379⟩. ⟨hal-03152386⟩

Share

Metrics

Record views

20

Files downloads

16