Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos - Sorbonne Université Access content directly
Journal Articles Nature Communications Year : 2021

Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos

Abstract

Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C. elegans embryos inherit a complementary pool of small non-coding RNAs that facilitate the cleavage and removal of hundreds of maternal mRNAs. These antisense small RNAs are loaded into the maternal catalytically-active Argonaute CSR-1 and cleave complementary mRNAs no longer engaged in translation in somatic blastomeres. Induced depletion of CSR-1 specifically during embryonic development leads to embryonic lethality in a slicer-dependent manner and impairs the degradation of CSR-1 embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operates to clear maternal mRNAs during the maternal-to-zygotic transition across species.
Fichier principal
Vignette du fichier
s41467-021-21691-6.pdf (3.11 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-03163367 , version 1 (09-03-2021)

Licence

Attribution

Identifiers

Cite

Piergiuseppe Quarato, Meetali Singh, Eric Cornes, Blaise Maxime Li, Loan Bourdon, et al.. Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos. Nature Communications, 2021, 12 (1), pp.1441. ⟨10.1038/s41467-021-21691-6⟩. ⟨hal-03163367⟩
59 View
60 Download

Altmetric

Share

Gmail Facebook X LinkedIn More