Myod1 and GR coordinate myofiber-specific transcriptional enhancers - Sorbonne Université
Article Dans Une Revue Nucleic Acids Research Année : 2021

Myod1 and GR coordinate myofiber-specific transcriptional enhancers

Scott A Jelinsky
  • Fonction : Auteur

Résumé

Skeletal muscle is a dynamic tissue the size of which can be remodeled through the concerted actions of various cues. Here, we investigated the skeletal muscle transcriptional program and identified key tissue-specific regulatory genetic elements. Our results show that Myod1 is bound to numerous skeletal muscle enhancers in collaboration with the glucocorticoid receptor (GR) to control gene expression. Remarkably, transcriptional activation controlled by these factors occurs through direct contacts with the promoter region of target genes, via the CpG-bound transcription factor Nrf1, and the formation of Ctcfanchored chromatin loops, in a myofiber-specific manner. Moreover, we demonstrate that GR negatively controls muscle mass and strength in mice by down-regulating anabolic pathways. Taken together, our data establish Myod1, GR and Nrf1 as key players of muscle-specific enhancer-promoter communication that orchestrate myofiber size regulation.
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Dates et versions

hal-03251776 , version 1 (07-06-2021)

Identifiants

Citer

Daniela Rovito, Anna-Isavella Rerra, Vanessa Ueberschlag-Pitiot, Shilpy Joshi, Nezih Karasu, et al.. Myod1 and GR coordinate myofiber-specific transcriptional enhancers. Nucleic Acids Research, 2021, 49 (8), pp.4472 - 4492. ⟨10.1093/nar/gkab226⟩. ⟨hal-03251776⟩
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