Fat inclusions strongly alter membrane mechanics - Sorbonne Université Access content directly
Journal Articles Biophysical Journal Year : 2021

Fat inclusions strongly alter membrane mechanics


Neutral lipids (NLs) are apolar oil molecules synthesized in the endoplasmic reticulum bilayer upon diverse biological stimuli. NLs synthesized are released in the hydrophobic core of the bilayer. At a critical concentration, NLs condense by phase separation and nucleate a lipid droplet (LD). After an LD forms, a fraction of NLs can be present in the bilayer but at a concentration below that of the nucleation. Here, we study whether and how the accumulation of NLs alters a lipid bilayer's mechanical properties. In synthetic systems, we found that NLs proffer unusual bilayer stretching capacities, especially in the presence of negatively curved phospholipids. This impact becomes spectacular when an LD is contiguous with the bilayer and supplies it with NLs. The tested NLs markedly decrease the bilayer area expansion modulus and significantly increase lysis tension but had opposite effects on membrane bending rigidity. Our data unveil how NL molecules modify overall membrane mechanics, the alteration of which may be linked to pathologies or anticancer treatments targeting NLs.
Fichier principal
Vignette du fichier
Santinho et al. - 2021 - Fat inclusions strongly alter membrane mechanics.pdf (6.17 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03267078 , version 1 (22-06-2021)



Alexandre Santinho, Aymeric Chorlay, Lionel Foret, Abdou Rachid Thiam. Fat inclusions strongly alter membrane mechanics. Biophysical Journal, 2021, 120 (4), pp.607-617. ⟨10.1016/j.bpj.2021.01.009⟩. ⟨hal-03267078⟩
46 View
63 Download



Gmail Mastodon Facebook X LinkedIn More