OVX836 Heptameric Nucleoprotein Vaccine Generates Lung Tissue-Resident Memory CD8+ T-Cells for Cross-Protection Against Influenza - Sorbonne Université
Article Dans Une Revue Frontiers in Immunology Année : 2021

OVX836 Heptameric Nucleoprotein Vaccine Generates Lung Tissue-Resident Memory CD8+ T-Cells for Cross-Protection Against Influenza

Judith del Campo
  • Fonction : Auteur
Julien Bouley
  • Fonction : Auteur
Marion Chevandier
  • Fonction : Auteur
Carine Rousset
  • Fonction : Auteur
Marjorie Haller
  • Fonction : Auteur
Alice Indalecio
  • Fonction : Auteur
Delphine Guyon-Gellin
  • Fonction : Auteur
Alexandre Le Vert
  • Fonction : Auteur
Fergal Hill
  • Fonction : Auteur
Yann Leverrier
  • Fonction : Auteur
Jacqueline Marvel
Florence Nicolas
  • Fonction : Auteur

Résumé

Tissue-resident memory (TRM) CD8+ T-cells play a crucial role in the protection against influenza infection but remain difficult to elicit using recombinant protein vaccines. OVX836 is a recombinant protein vaccine, obtained by the fusion of the DNA sequence of the influenza A nucleoprotein (NP) to the DNA sequence of the OVX313 heptamerization domain. We previously demonstrated that OVX836 provides broad-spectrum protection against influenza viruses. Here, we show that OVX836 intramuscular (IM) immunization induces higher numbers of NP-specific IFNg-producing CD8+ T-cells in the lung, compared to mutant NP (NPm) and wild-type NP (NPwt), which form monomeric and trimeric structures, respectively. OVX836 induces cytotoxic CD8+ T-cells and high frequencies of lung TRM CD8+ T-cells, while inducing solid protection against lethal influenza virus challenges for at least 90 days. Adoptive transfer experiments demonstrated that protection against diverse influenza subtypes is mediated by NPspecific CD8+ T-cells isolated from the lung and spleen following OVX836 vaccination. OVX836 induces a high number of NP-specific lung CD8+ TRM-cells for long-term protection against influenza viruses.

Domaines

Immunologie
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Dates et versions

hal-03278552 , version 1 (05-07-2021)

Identifiants

Citer

Judith del Campo, Julien Bouley, Marion Chevandier, Carine Rousset, Marjorie Haller, et al.. OVX836 Heptameric Nucleoprotein Vaccine Generates Lung Tissue-Resident Memory CD8+ T-Cells for Cross-Protection Against Influenza. Frontiers in Immunology, 2021, 12, ⟨10.3389/fimmu.2021.678483⟩. ⟨hal-03278552⟩
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