Cytokine receptor cluster size impacts its endocytosis and signaling - Sorbonne Université
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2021

Cytokine receptor cluster size impacts its endocytosis and signaling

Abstract

The interleukin-2 receptor (IL-2R) is a cytokine receptor essential for immunity that transduces proliferative signals regulated by its uptake and degradation. IL-2R is a well-known marker of clathrin-independent endocytosis (CIE), a process devoid of any coat protein, raising the question of how the CIE vesicle is generated. Here, we investigated the impact of IL-2Rγ clustering in its endocytosis. Combining total internal reflection fluorescence (TIRF) live imaging of a CRISPR-edited T cell line endogenously expressing IL-2Rγ tagged with green fluorescent protein (GFP), with multichannel imaging, single-molecule tracking, and quantitative analysis, we were able to decipher IL-2Rγ stoichiometry at the plasma membrane in real time. We identified three distinct IL-2Rγ cluster populations. IL-2Rγ is secreted to the cell surface as a preassembled small cluster of three molecules maximum, rapidly diffusing at the plasma membrane. A medium-sized cluster composed of four to six molecules is key for IL-2R internalization and is promoted by interleukin 2 (IL-2) binding, while larger clusters (more than six molecules) are static and inefficiently internalized. Moreover, we identified membrane cholesterol and the branched actin cytoskeleton as key regulators of IL-2Rγ clustering and IL-2–induced signaling. Both cholesterol depletion and Arp2/3 inhibition lead to the assembly of large IL-2Rγ clusters, arising from the stochastic interaction of receptor molecules in close correlation with their enhanced lateral diffusion at the membrane, thus resulting in a default in IL-2R endocytosis. Despite similar clustering outcomes, while cholesterol depletion leads to a sustained IL-2–dependent signaling, Arp2/3 inhibition prevents signal initiation. Taken together, our results reveal the importance of cytokine receptor clustering for CIE initiation and signal transduction.

Dates and versions

hal-03352957 , version 1 (23-09-2021)

Identifiers

Cite

Laura Salavessa, Thibault Lagache, Valérie Malardé, Alexandre Grassart, Jean-Christophe Olivo-Marin, et al.. Cytokine receptor cluster size impacts its endocytosis and signaling. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (37), pp.e2024893118. ⟨10.1073/pnas.2024893118⟩. ⟨hal-03352957⟩
79 View
0 Download

Altmetric

Share

More