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Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors

Abstract : The architecturally stereotypical structure of cerebellum is ideal for investigating the generation of neuronal diversity, but in vitro models for assessing early cerebellar progenitor differentiation were lacking. Here, we report a detailed protocol for long-term in vitro generation of Pax6+ granule cells and Calbindin+ Purkinje cells from common Sox2+ embryonic cerebellar progenitors. We describe the procedure for dissecting mouse cerebellar anlage, cell seeding, and tamoxifen-induced labeling of progenitor cells, followed by time-lapse video recording of clonal expansion and neuronal differentiation.
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Submitted on : Thursday, September 23, 2021 - 4:05:20 PM
Last modification on : Friday, September 24, 2021 - 10:34:37 AM

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Tingting Zhang, Tengyuan Liu, Bassem Hassan. Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors. STAR Protocols, Elsevier, 2021, 2 (3), pp.100760. ⟨10.1016/j.xpro.2021.100760⟩. ⟨hal-03352961⟩

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