A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure - Sorbonne Université Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2021

A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure

Résumé

Fucoidans are sulfated, fucose-rich marine polysaccharides primarily found in cell walls of brown seaweeds (macroalgae). Fucoidans are known to possess beneficial bioactivities depending on their structure and sulfation degree. Here, we report the first functional characterization and the first crystal structure of a prokaryotic sulfatase, PsFucS1, belonging to sulfatase subfamily S1_13, able to release sulfate from fucoidan oligosaccharides. PsFucS1 was identified in the genome of a Pseudoalteromonas sp. isolated from sea cucumber gut. PsFucS1 (57 kDa) is Ca2+ dependent and has an unusually high optimal temperature (68 °C) and thermostability. Further, the PsFucS1 displays a unique quaternary hexameric structure comprising a tight trimeric dimer complex. The structural data imply that this hexamer formation results from an uncommon interaction of each PsFucS1 monomer that is oriented perpendicular to the common dimer interface (~ 1500 Å2) that can be found in analogous sulfatases. The uncommon interaction involves interfacing (1246 Å2) through a bundle of α-helices in the N-terminal domain to form a trimeric ring structure. The high thermostability may be related to this unusual quaternary hexameric structure formation that is suggested to represent a novel protein thermostabilization mechanism.

Mots clés

Fichier principal
Vignette du fichier
s41598-021-98588-3.pdf (1.9 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

hal-03367940 , version 1 (06-10-2021)

Identifiants

Citer

Maria Dalgaard Mikkelsen, Hang Thi Thuy Cao, Thomas Roret, Nanna Rhein-Knudsen, Jesper Holck, et al.. A novel thermostable prokaryotic fucoidan active sulfatase PsFucS1 with an unusual quaternary hexameric structure. Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-98588-3⟩. ⟨hal-03367940⟩
82 Consultations
67 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More