Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology - Sorbonne Université
Journal Articles Nature Communications Year : 2021

Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology

Edward A Hayter
Jason Day
Gerben van Ooijen
David A Bechtold
Peter Newham

Abstract

Between 6–20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na + , K + , and Cl − through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes.
Fichier principal
Vignette du fichier
s41467-021-25942-4.pdf (2.71 Mo) Télécharger le fichier
Origin Publication funded by an institution

Dates and versions

hal-03444276 , version 1 (23-11-2021)

Identifiers

Cite

Alessandra Stangherlin, Joseph L Watson, David C S Wong, Silvia Barbiero, Aiwei Zeng, et al.. Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology. Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-021-25942-4⟩. ⟨hal-03444276⟩
50 View
108 Download

Altmetric

Share

More