Topology of the redox network during induction of photosynthesis as revealed by time-resolved proteomics in tobacco - Sorbonne Université
Article Dans Une Revue Science Advances Année : 2021

Topology of the redox network during induction of photosynthesis as revealed by time-resolved proteomics in tobacco

Résumé

Photosynthetically produced electrons provide energy for various metabolic pathways, including carbon reduction. Four Calvin-Benson cycle enzymes and several other plastid proteins are activated in the light by reduction of specific cysteines via thioredoxins, a family of electron transporters operating in redox regulation networks. How does this network link the photosynthetic chain with cellular metabolism? Using a time-resolved redox proteomic method, we have investigated the redox network in vivo during the dark-to-low light transition. We show that redox states of some thioredoxins follow the photosynthetic linear electron transport rate. While some redox targets have kinetics compatible with an equilibrium with one thioredoxin (TRXf), reduction of other proteins shows specific kinetic limitations, allowing fine-tuning of each redox-regulated step of chloroplast metabolism. We identified five new redox-regulated proteins, including proteins involved in Mg 2+ transport and 1 O 2 signaling. Our results provide a system-level functional view of the photosynthetic redox regulation network.
Fichier principal
Vignette du fichier
sciadv.abi8307.pdf (2.38 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03499568 , version 1 (21-12-2021)

Licence

Identifiants

Citer

David Zimmer, Corné Swart, Alexander Graf, Stéphanie Arrivault, Michael Tillich, et al.. Topology of the redox network during induction of photosynthesis as revealed by time-resolved proteomics in tobacco. Science Advances , 2021, 7 (51), pp.eabi8307. ⟨10.1126/sciadv.abi8307⟩. ⟨hal-03499568⟩
35 Consultations
179 Téléchargements

Altmetric

Partager

More