The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1 - Sorbonne Université
Article Dans Une Revue BMC Biology Année : 2022

The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1

Chang Xie
  • Fonction : Auteur
Xin Wei
  • Fonction : Auteur
  • PersonId : 772620
  • IdRef : 181647419
Yue Gao
  • Fonction : Auteur
Jing Yao
  • Fonction : Auteur
  • PersonId : 1092201

Résumé

Background: The synthetic chemical 1,4-dioxane is used as industrial solvent, food, and care product additive. 1,4-Dioxane has been noted to influence the nervous system in long-term animal experiments and in humans, but the molecular mechanisms underlying its effects on animals were not previously known. Results: Here, we report that 1,4-dioxane potentiates the capsaicin-sensitive transient receptor potential (TRP) channel TRPV1, thereby causing hyperalgesia in mouse model. This effect was abolished by CRISPR/Cas9-mediated genetic deletion of TRPV1 in sensory neurons, but enhanced under inflammatory conditions. 1,4-Dioxane lowered the temperature threshold for TRPV1 thermal activation and potentiated the channel sensitivity to agonistic stimuli. 1,3-dioxane and tetrahydrofuran which are structurally related to 1,4-dioxane also potentiated TRPV1 activation. The residue M572 in the S4-S5 linker region of TRPV1 was found to be crucial for direct activation of the channel by 1,4dioxane and its analogs. A single residue mutation M572V abrogated the 1,4-dioxane-evoked currents while largely preserving the capsaicin responses. Our results further demonstrate that this residue exerts a gating effect through hydrophobic interactions and support the existence of discrete domains for multimodal gating of TRPV1 channel. Conclusions: Our results suggest TRPV1 is a co-receptor for 1,4-dioxane and that this accounts for its ability to dysregulate body nociceptive sensation.

Domaines

Immunologie
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Dates et versions

hal-03519204 , version 1 (10-01-2022)

Identifiants

Citer

Xiaoyi Mo, Qiang Liu, Luna Gao, Chang Xie, Xin Wei, et al.. The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1. BMC Biology, 2022, 20 (1), pp.10. ⟨10.1186/s12915-021-01211-0⟩. ⟨hal-03519204⟩
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