Accurate determination of marker location within whole-brain microscopy images - Sorbonne Université
Journal Articles Scientific Reports Year : 2022

Accurate determination of marker location within whole-brain microscopy images

Abstract

High-resolution whole-brain microscopy provides a means for post hoc determination of the location of implanted devices and labelled cell populations that are necessary to interpret in vivo experiments designed to understand brain function. Here we have developed two plugins (brainreg and brainreg-segment) for the Python-based image viewer napari, to accurately map any object in a common coordinate space. We analysed the position of dye-labelled electrode tracks and two-photon imaged cell populations expressing fluorescent proteins. The precise location of probes and cells were physiologically interrogated and revealed accurate segmentation with near-cellular resolution.
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Dates and versions

hal-03537546 , version 1 (20-01-2022)

Identifiers

Cite

Adam L. Tyson, Mateo Vélez-Fort, Charly V. Rousseau, Lee Cossell, Chryssanthi Tsitoura, et al.. Accurate determination of marker location within whole-brain microscopy images. Scientific Reports, 2022, 12 (1), pp.867. ⟨10.1038/s41598-021-04676-9⟩. ⟨hal-03537546⟩
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