A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis - Sorbonne Université Access content directly
Journal Articles Med Year : 2021

A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis

Pedro Goncalves
James P. Di Santo
Hugo Mouquet
Olivier Schwartz
Aurélien Corneau
Damien Bonnet
Pediatric-Biocovid Study Group
  • Function : Author

Abstract

BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children is generally milder than in adults, but a proportion of cases result in hyperinflammatory conditions often including myocarditis.MethodsTo better understand these cases, we applied a multiparametric approach to the study of blood cells of 56 children hospitalized with suspicion of SARS-CoV-2 infection. Plasma cytokine and chemokine levels and blood cellular composition were measured, alongside gene expression at the bulk and single-cell levels.FindingsThe most severe forms of multisystem inflammatory syndrome in children (MIS-C) related to SARS-CoV-2 that resulted in myocarditis were characterized by elevated levels of pro-angiogenesis cytokines and several chemokines. Single-cell transcriptomics analyses identified a unique monocyte/dendritic cell gene signature that correlated with the occurrence of severe myocarditis characterized by sustained nuclear factor κB (NF-κB) activity and tumor necrosis factor alpha (TNF-α) signaling and associated with decreased gene expression of NF-κB inhibitors. We also found a weak response to type I and type II interferons, hyperinflammation, and response to oxidative stress related to increased HIF-1α and Vascular endothelial growth factor (VEGF) signaling.ConclusionsThese results provide potential for a better understanding of disease pathophysiology.
Fichier principal
Vignette du fichier
S2666634021002877.pdf (108.65 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03590894 , version 1 (16-10-2023)

Licence

Identifiers

Cite

Camille de Cevins, Marine Luka, Nikaïa Smith, Sonia Meynier, Aude Magérus, et al.. A monocyte/dendritic cell molecular signature of SARS-CoV-2-related multisystem inflammatory syndrome in children with severe myocarditis. Med, 2021, 2 (9), pp.1072-1092.e7. ⟨10.1016/j.medj.2021.08.002⟩. ⟨hal-03590894⟩
84 View
4 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More