Activity-dependent inhibitory synapse scaling is determined by gephyrin phosphorylation and subsequent regulation of GABAA receptor diffusion - Sorbonne Université
Article Dans Une Revue eNeuro Année : 2018

Activity-dependent inhibitory synapse scaling is determined by gephyrin phosphorylation and subsequent regulation of GABAA receptor diffusion

Sereina Battaglia
  • Fonction : Auteur
Marianne Renner
  • Fonction : Auteur
Marion Russeau
  • Fonction : Auteur
Etienne Côme
  • Fonction : Auteur
K. Tyagarajan Shiva
  • Fonction : Auteur
Sabine Lévi
Connectez-vous pour contacter l'auteur

Résumé

Synaptic plasticity relies on the rapid changes in neurotransmitter receptor number at postsynaptic sites. Using super resolution PALM imaging and quantum-dot based single particle tracking in rat hippocampal cultured neurons, we investigated if the phosphorylation status of the main scaffolding protein gephyrin influenced the organization of the gephyrin scaffold and GABAA receptor (GABAAR) membrane dynamics. We found that gephyrin phosphorylation regulates gephyrin microdomain compaction. The ERK1/2 and GSK3 signaling alter the gephyrin scaffold mesh differentially. Differences in scaffold organization impacted similarly the diffusion of synaptic GABAARs, suggesting reduced gephyrin-receptor binding properties. In the context of synaptic scaling, our results identify a novel role of the GSK3 signaling pathway in the activity-dependent regulation of extrasynaptic receptor surface trafficking and GSK3PKA and CaMKII pathways in facilitating adaptations of synaptic receptors.

Domaines

Neurobiologie
Fichier principal
Vignette du fichier
Battaglia et al.eNeuro-last.pdf (3.3 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03977654 , version 1 (07-02-2023)

Identifiants

Citer

Sereina Battaglia, Marianne Renner, Marion Russeau, Etienne Côme, K. Tyagarajan Shiva, et al.. Activity-dependent inhibitory synapse scaling is determined by gephyrin phosphorylation and subsequent regulation of GABAA receptor diffusion. eNeuro, 2018, ⟨10.1523/ENEURO.0203-17.2017⟩. ⟨hal-03977654⟩
15 Consultations
18 Téléchargements

Altmetric

Partager

More