Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish - Sorbonne Université Access content directly
Journal Articles Development (Cambridge, England) Year : 2017

Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish

Ming Shao
  • Function : Author
Min Wang
  • Function : Author
Yuan-Yuan Liu
  • Function : Author
Yi-Wen Ge
  • Function : Author
Yan-Jun Zhang
  • Function : Author
  • PersonId : 1231323

Abstract

The vegetal pole cytoplasm represents a crucial source of maternal dorsal determinants for patterning the dorsoventral axis of the early embryo. Removal of the vegetal yolk in the zebrafish fertilised egg before the completion of the first cleavage results in embryonic ventralisation, but removal of this part at the two-cell stage leads to embryonic dorsalisation. How this is achieved remains unknown. Here, we report a novel mode of maternal regulation of BMP signalling during dorsoventral patterning in zebrafish. We identify Vrtn as a novel vegetally localised maternal factor with dorsalising activity and rapid transport towards the animal pole region after fertilisation. Co-injection of vrtn mRNA with vegetal RNAs from different cleavage stages suggests the presence of putative vegetally localised Vrtn antagonists with slower animal pole transport. Thus, vegetal ablation at the two-cell stage could remove most of the Vrtn antagonists, and allows Vrtn to produce the dorsalising effect. Mechanistically, Vrtn binds a bmp2b regulatory sequence and acts as a repressor to inhibit its zygotic transcription. Analysis of maternal-zygotic vrtn mutants further shows that Vrtn is required to constrain excessive bmp2b expression in the margin. Our work unveils a novel maternal mechanism regulating zygotic BMP gradient in dorsoventral patterning.
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hal-04004478 , version 1 (06-03-2023)

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Ming Shao, Min Wang, Yuan-Yuan Liu, Yi-Wen Ge, Yan-Jun Zhang, et al.. Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish. Development (Cambridge, England), 2017, 144 (18), pp.3361-3374. ⟨10.1242/dev.152553⟩. ⟨hal-04004478⟩
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