N-Oleoyl dopamine induces IL-10 via central nervous system TRPV1 and improves endotoxemia and sepsis outcomes - Sorbonne Université Access content directly
Journal Articles Journal of Neuroinflammation Year : 2022

N-Oleoyl dopamine induces IL-10 via central nervous system TRPV1 and improves endotoxemia and sepsis outcomes

Erika Wong
  • Function : Author
Samira Lawton
  • Function : Author
Elliot Lloyd
  • Function : Author
Nina Nguyen
  • Function : Author
Fengyun Xu
  • Function : Author
Cristina Sempio
  • Function : Author
Lester Kobzik
  • Function : Author
Ivana Zlatanova
  • Function : Author
Mark Schumacher
  • Function : Author
Jost Klawitter
  • Function : Author
Hua Su
  • Function : Author
Katalin Rabl
  • Function : Author
Kevin Wilhelmsen
  • Function : Author
Che-Chung Yeh
  • Function : Author
Judith Hellman

Abstract

Background: The transient receptor potential vanilloid 1 (TRPV1) participates in thermosensation and inflammatory pain, but its immunomodulatory mechanisms remain enigmatic. N-Oleoyl dopamine (OLDA), an endovanilloid and endocannabinoid, is a TRPV1 agonist that is produced in the central nervous system and the peripheral nervous system. We studied the anti-inflammatory effects and TRPV1-dependent mechanisms of OLDA in models of inflammation and sepsis. Methods: Mice were challenged intratracheally or intravenously with LPS, or intratracheally with S. aureus to induce pneumonia and sepsis, and then were treated intravenously with OLDA. Endpoints included plasma cytokines, leukocyte activation marker expression, mouse sepsis scores, lung histopathology, and bacterial counts. The role of TRPV1 in the effects of OLDA was determined using Trpv1 −/− mice, and mice with TRPV1 knockdown pan-neuronally, in peripheral nervous system neurons, or in myeloid cells. Circulating monocytes/macrophages were depleted using clodronate to determine their role in the anti-inflammatory effects of OLDA in endotoxemic mice. Levels of exogenous OLDA, and of endovanilloids and endocannabinoids, at baseline and in endotoxemic mice, were determined by LC-MS/MS. Results: OLDA administration caused an early anti-inflammatory response in endotoxemic and septic mice with high serum levels of IL-10 and decreased levels of pro-inflammatory cytokines. OLDA also reduced lung injury and improved mouse sepsis scores. Blood and lung bacterial counts were comparable between OLDA-and carrier-treated mice with S. aureus pneumonia. OLDA's effects were reversed in mice with pan-neuronal TRPV1 knockdown, but not with TRPV1 knockdown in peripheral nervous system neurons or myeloid cells. Depletion of monocytes/macrophages reversed the IL-10 upregulation by OLDA in endotoxemic mice. Brain and blood levels of endovanilloids and endocannabinoids were increased in endotoxemic mice. Conclusions: OLDA has strong anti-inflammatory actions in mice with endotoxemia or S. aureus pneumonia. Prior studies focused on the role of peripheral nervous system TRPV1 in modulating inflammation and pneumonia. Our results suggest that TRPV1-expressing central nervous system neurons also regulate inflammatory responses to endotoxemia and infection. Our study reveals a neuro-immune reflex that during acute inflammation is engaged
Fichier principal
Vignette du fichier
12974_2022_Article_2485.pdf (4.26 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive

Dates and versions

hal-04022304 , version 1 (09-03-2023)

Identifiers

Cite

Jérémie Joffre, Erika Wong, Samira Lawton, Elliot Lloyd, Nina Nguyen, et al.. N-Oleoyl dopamine induces IL-10 via central nervous system TRPV1 and improves endotoxemia and sepsis outcomes. Journal of Neuroinflammation, 2022, 19, ⟨10.1186/s12974-022-02485-z⟩. ⟨hal-04022304⟩
7 View
7 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More