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Journal Articles Scientific Reports Year : 2023

A simple method to make, trap and deform a vesicle in a gel

Abstract

Abstract We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of α -Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.
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Dates and versions

hal-04062537 , version 1 (07-04-2023)

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Pierre Tapie, Alexis Prevost, Lorraine Montel, Léa-Laetitia Pontani, Elie Wandersman. A simple method to make, trap and deform a vesicle in a gel. Scientific Reports, 2023, 13 (1), pp.5375. ⟨10.1038/s41598-023-31996-9⟩. ⟨hal-04062537⟩
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