Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae) - Sorbonne Université Access content directly
Journal Articles Biosensors and Bioelectronics Year : 2006

Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae)

Abstract

We report the immobilization on a gold surface of a 20-base DNA probe labeled with disulfide group and on the selective hybridization with the complementary 20-base DNA strand. The oligonucleotide probe is the complementary strand of a partial sequence of the gene encoding for a large ribosomal RNA sub-unit which is a coding sequence of Alexandrium minutum DNA, a microalgae that produces neurotoxins responsible for paralytic shellfish poisoning on European and Asian coasts. The kinetics of DNA probe immobilization and hybridization were monitored in situ by using a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the setup is stable to within a few hertz, corresponding to the nanogram scale, for 3 h and makes it possible to follow frequency change from immobilization of the probe to hybridization of the complementary DNA target. This setup constitutes a biosensor, which is sensitive and selective, and the hybridization ratio between hybridized complementary DNA and immobilized DNA probes is 47%.

Dates and versions

hal-04165600 , version 1 (19-07-2023)

Identifiers

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Mathieu Lazerges, Hubert Perrot, E. Antoine, A. Defontaine, Chantal Compere. Oligonucleotide quartz crystal microbalance sensor for the microalgae Alexandrium minutum (Dinophyceae). Biosensors and Bioelectronics, 2006, 21 (7), pp.1355-1358. ⟨10.1016/j.bios.2005.05.013⟩. ⟨hal-04165600⟩
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