In situ QCM DNA-biosensor probe modification - Sorbonne Université Access content directly
Journal Articles Sensors and Actuators B: Chemical Year : 2006

In situ QCM DNA-biosensor probe modification

Abstract

We report on a probe modification of a quartz crystal microbalance (QCM) DNA-biosensor that permits to reversibly change the DNA sequence detected. A QCM DNA-biosensor was designed by immobilization of a 20-base DNA-disulfide probe on the gold-covered quartz surface of a 27 MHz microbalance (9 MHz, third overtone). After immobilization on the gold covered quartz surface, this probe was modified by hybridization with a 45-base DNA that includes the complementary 20-base sequence, a 5-base spacer and a non-complementary 20-base sequence. The non-complementary sequence constitutes a new probe, different from the DNA-disulfide probe, that permits the detection of a new DNA target. As this 45 bases DNA is changeable by dehybridization, successive different DNA targets can be detected. Kinetics and thermodynamic studies of the DNA-disulfide and modified biosensors indicate that the modified biosensor is as sensitive, selective, fast, renewable and reproducible as the DNA-disulfide biosensor, but with a higher hybridization ratio. This modification method offers wider investigation field and practical and economic advantages to DNA-biosensors based on irreversible immobilization of DNA probes on solid substrate. (c) 2006 Elsevier B.V. All rights reserved.
Fichier principal
Vignette du fichier
QCM-DNA_2006.pdf (185.08 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-04171258 , version 1 (26-07-2023)

Identifiers

Cite

Mathieu Lazerges, Hubert Perrot, N. Zeghib, E. Antoine, Chantal Compere. In situ QCM DNA-biosensor probe modification. Sensors and Actuators B: Chemical, 2006, 120 (1), pp.329-337. ⟨10.1016/j.snb.2006.02.024⟩. ⟨hal-04171258⟩
3 View
14 Download

Altmetric

Share

Gmail Facebook X LinkedIn More