Proteasome inhibition alters mitotic progression through the upregulation of centromeric α‐Satellite RNAs - Sorbonne Université
Article Dans Une Revue FEBS Journal Année : 2022

Proteasome inhibition alters mitotic progression through the upregulation of centromeric α‐Satellite RNAs

Rodrigo Cáceres-Gutiérrez
  • Fonction : Auteur
Marco Andonegui
  • Fonction : Auteur
Diego Oliva-Rico
  • Fonction : Auteur
Rodrigo González-Barrios
  • Fonction : Auteur
Fernando Luna
  • Fonction : Auteur
Cristian Arriaga-Canon
  • Fonction : Auteur
Alejandro López-Saavedra
  • Fonction : Auteur
Diddier Prada
  • Fonction : Auteur
Clementina Castro
  • Fonction : Auteur
Laurent Parmentier
  • Fonction : Auteur
José Díaz-Chávez
  • Fonction : Auteur
Yair Alfaro-Mora
  • Fonction : Auteur
Erick Navarro-Delgado
  • Fonction : Auteur
Eunice Fabian-Morales
  • Fonction : Auteur
Bao Tran
  • Fonction : Auteur
Jyoti Shetty
  • Fonction : Auteur
Yongmei Zhao
  • Fonction : Auteur
Nicolas Alcaraz
  • Fonction : Auteur
Carlos de la Rosa
  • Fonction : Auteur
José Reyes
  • Fonction : Auteur
Sabrine Hédouin
  • Fonction : Auteur
Claire Francastel
  • Fonction : Auteur
Luis Herrera

Résumé

Cell cycle progression requires control of the abundance of several proteins and RNAs over space and time to properly transit from one phase to the next and to ensure faithful genomic inheritance in daughter cells. The proteasome, the main protein degradation system of the cell, facilitates the establishment of a proteome specific to each phase of the cell cycle. Its activity also strongly influences transcription. Here, we detected the upregulation of repetitive RNAs upon proteasome inhibition in human cancer cells using RNA‐seq. The effect of proteasome inhibition on centromeres was remarkable, especially on α‐Satellite RNAs. We showed that α‐Satellite RNAs fluctuate along the cell cycle and interact with members of the cohesin ring, suggesting that these transcripts may take part in the regulation of mitotic progression. Next, we forced exogenous overexpression and used gapmer oligonucleotide targeting to demonstrate that α‐Sat RNAs have regulatory roles in mitosis. Finally, we explored the transcriptional regulation of α‐Satellite DNA. Through in silico analyses, we detected the presence of CCAAT transcription factor‐binding motifs within α‐Satellite centromeric arrays. Using high‐resolution three‐dimensional immuno‐FISH and ChIP‐qPCR, we showed an association between the α‐Satellite upregulation and the recruitment of the transcription factor NFY‐A to the centromere upon MG132‐induced proteasome inhibition. Together, our results show that the proteasome controls α‐Satellite RNAs associated with the regulation of mitosis.

Dates et versions

hal-04273186 , version 1 (07-11-2023)

Identifiants

Citer

Rodrigo Cáceres-Gutiérrez, Marco Andonegui, Diego Oliva-Rico, Rodrigo González-Barrios, Fernando Luna, et al.. Proteasome inhibition alters mitotic progression through the upregulation of centromeric α‐Satellite RNAs. FEBS Journal, 2022, 289 (7), pp.1858-1875. ⟨10.1111/febs.16261⟩. ⟨hal-04273186⟩
14 Consultations
0 Téléchargements

Altmetric

Partager

More