Transcriptional diversity in specific synaptic gene sets discriminates cortical neuronal identity - Sorbonne Université Access content directly
Journal Articles Biology Direct Year : 2023

Transcriptional diversity in specific synaptic gene sets discriminates cortical neuronal identity

Tilmann Achsel
Maria Andres-Alonso
Claudia Bagni
Àlex Bayés
Thomas Biederer
Nils Brose
John Jia En Chua
Marcelo P Coba
L. Niels Cornelisse
Jaime de Juan-Sanz
Hana L Goldschmidt
Eckart D Gundelfinger
Richard L Huganir
Cordelia Imig
Reinhard Jahn
Hwajin Jung
Pascal S Kaeser
Eunjoon Kim
Frank Koopmans
Michael R Kreutz
Noa Lipstein
Harold D Macgillavry
Peter S Mcpherson
Vincent O’connor
Rainer Pielot
Timothy A Ryan
Carlo Sala
Morgan Sheng
Karl-Heinz Smalla
Paul D Thomas
Ruud F Toonen
Jan R T van Weering
Chiara Verpelli

Abstract

Synapse diversity has been described from different perspectives, ranging from the specific neurotransmitters released, to their diverse biophysical properties and proteome profiles. However, synapse diversity at the transcriptional level has not been systematically identified across all synapse populations in the brain. To quantify and identify specific synaptic features of neuronal cell types we combined the SynGO (Synaptic Gene Ontology) database with single-cell RNA sequencing data of the mouse neocortex. We show that cell types can be discriminated by synaptic genes alone with the same power as all genes. The cell type discriminatory power is not equally distributed across synaptic genes as we could identify functional categories and synaptic compartments with greater cell type specific expression. Synaptic genes, and specific SynGO categories, belonged to three different types of gene modules: gradient expression over all cell types, gradient expression in selected cell types and cell class- or type-specific profiles. This data provides a deeper understanding of synapse diversity in the neocortex and identifies potential markers to selectively identify synapses from specific neuronal populations.
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hal-04540396 , version 1 (10-04-2024)

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Amparo Roig Adam, José A Martínez-López, Sophie J F van der Spek, Patrick F Sullivan, August B Smit, et al.. Transcriptional diversity in specific synaptic gene sets discriminates cortical neuronal identity. Biology Direct, 2023, 18, pp.22. ⟨10.1186/s13062-023-00372-y⟩. ⟨hal-04540396⟩
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