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Journal Articles Nature Communications Year : 2018

Microfluidic active loading of single cells enables analysis of complex clinical specimens

Abstract

A fundamental trade-off between flow rate and measurement precision limits performance of many single-cell detection strategies, especially for applications that require biophysical measurements from living cells within complex and low-input samples. To address this, we introduce ‘active loading’, an automated, optically-triggered fluidic system that improves measurement throughput and robustness by controlling entry of individual cells into a measurement channel. We apply active loading to samples over a range of concentrations (1–1000 particles μL −1 ), demonstrate that measurement time can be decreased by up to 20-fold, and show theoretically that performance of some types of existing single-cell microfluidic devices can be improved by implementing active loading. Finally, we demonstrate how active loading improves clinical feasibility for acute, single-cell drug sensitivity measurements by deploying it to a preclinical setting where we assess patient samples from normal brain, primary and metastatic brain cancers containing a complex, difficult-to-measure mixture of confounding biological debris.
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Origin : Publication funded by an institution
Licence : CC BY - Attribution

Dates and versions

hal-04540842 , version 1 (10-04-2024)

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Nicholas L Calistri, Robert J Kimmerling, Seth W Malinowski, Mehdi Touat, Mark M Stevens, et al.. Microfluidic active loading of single cells enables analysis of complex clinical specimens. Nature Communications, 2018, 9 (1), pp.4784. ⟨10.1038/s41467-018-07283-x⟩. ⟨hal-04540842⟩
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