The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue - Sorbonne Université Access content directly
Journal Articles Nature Methods Year : 2019

The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Fabian Voigt
Daniel Kirschenbaum
  • Function : Author
Evgenia Platonova
  • Function : Author
Robert Campbell
  • Function : Author
Rahel Kastli
Ladan Egolf
  • Function : Author
Karen Haenraets
Thomas Topilko
Paola Perin
Sven Hildebrand
Anna Schueth
  • Function : Author
Alard Roebroeck
  • Function : Author
Esther Stoeckli
Roberto Pizzala
Nicolas Renier
Hanns Ulrich Zeilhofer
Theofanis Karayannis
  • Function : Author
Urs Ziegler
  • Function : Author
Laura Batti
  • Function : Author
Anthony Holtmaat
  • Function : Author
Christian Lüscher
  • Function : Author
Adriano Aguzzi
Fritjof Helmchen

Abstract

Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).

Dates and versions

hal-04578933 , version 1 (17-05-2024)

Identifiers

Cite

Fabian Voigt, Daniel Kirschenbaum, Evgenia Platonova, Stéphane Pagès, Robert Campbell, et al.. The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue. Nature Methods, 2019, 16 (11), pp.1105-1108. ⟨10.1038/s41592-019-0554-0⟩. ⟨hal-04578933⟩
0 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More