Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection - Archive ouverte HAL Access content directly
Journal Articles Biomacromolecules Year : 2017

Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection


Gene transfection with polymeric carrier remains a challenge, particularly high transfection levels combined with low toxicity are hard to achieve. We herein revisit polyvinylamines, an old and neglected family of cationic polymers. They can be readily obtained by controlled hydrolysis of polyvinylamides prepared through (controlled) radical polymerization. A series of tailor-made and well-defined polyvinylamines bearing primary amino groups, and poly(N-methylvinylamine) bearing secondary amines, were evaluated for the transfection of cells with pDNA as a function of their molar mass, molar mass distribution and degree of deacetylation. Unexpected high transfection levels, in combination with low cytotoxicity were recorded for both series. Surprisingly, a great impact of the molar mass was observed for the primary amine polyvinylamine series, whereas the results were mostly independent on molar mass or dispersity for the polymer bearing secondary amine. It was further established that a certain percentage of acetamide groups increased the transfection level, while maintaining low cytotoxicity. These results highlight for the first time the real potential of polyvinylamines as gene carriers, and make these polymers very attractive for further development in gene therapy.
Fichier principal
Vignette du fichier
Drean_2016_Use_of_Primary_and.pdf (1.29 Mo) Télécharger le fichier
Biomacromolecules_Guegan_SI_revised_v3.pdf (791.22 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

hal-01427315 , version 1 (05-01-2017)



Mathilde Dréan, Antoine Debuigne, Cristine Gonçalves, Christine Jérôme, Patrick Midoux, et al.. Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection. Biomacromolecules, 2017, 18 (2), pp.440-451. ⟨10.1021/acs.biomac.6b01526⟩. ⟨hal-01427315⟩
116 View
461 Download



Gmail Facebook Twitter LinkedIn More