Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection

Abstract : Gene transfection with polymeric carrier remains a challenge, particularly high transfection levels combined with low toxicity are hard to achieve. We herein revisit polyvinylamines, an old and neglected family of cationic polymers. They can be readily obtained by controlled hydrolysis of polyvinylamides prepared through (controlled) radical polymerization. A series of tailor-made and well-defined polyvinylamines bearing primary amino groups, and poly(N-methylvinylamine) bearing secondary amines, were evaluated for the transfection of cells with pDNA as a function of their molar mass, molar mass distribution and degree of deacetylation. Unexpected high transfection levels, in combination with low cytotoxicity were recorded for both series. Surprisingly, a great impact of the molar mass was observed for the primary amine polyvinylamine series, whereas the results were mostly independent on molar mass or dispersity for the polymer bearing secondary amine. It was further established that a certain percentage of acetamide groups increased the transfection level, while maintaining low cytotoxicity. These results highlight for the first time the real potential of polyvinylamines as gene carriers, and make these polymers very attractive for further development in gene therapy.
Document type :
Journal articles
Complete list of metadatas

https://hal.sorbonne-universite.fr/hal-01427315
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Thursday, January 5, 2017 - 3:16:26 PM
Last modification on : Thursday, June 20, 2019 - 12:22:09 PM
Long-term archiving on : Thursday, April 6, 2017 - 2:07:36 PM

Identifiers

Citation

Mathilde Dréan, Antoine Debuigne, Cristine Gonçalves, Christine Jérôme, Patrick Midoux, et al.. Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection. Biomacromolecules, American Chemical Society, 2016, 18 (2), pp.440-451. ⟨10.1021/acs.biomac.6b01526⟩. ⟨hal-01427315⟩

Share

Metrics

Record views

207

Files downloads

261