A Genomic Survey of Reb Homologs Suggests Widespread Occurrence of R-Bodies in Proteobacteria

Abstract : Bacteria and eukaryotes are involved in many types of interaction in nature, with important ecological consequences. However, the diversity, occurrence, and mechanisms of these interactions often are not fully known. The obligate bacterial endosymbionts of Paramecium provide their hosts with the ability to kill sensitive Paramecium strains through the production of R-bodies, highly insoluble coiled protein ribbons. R-bodies have been observed in a number of free-living bacteria, where their function is unknown. We have performed an exhaustive survey of genes coding for homologs of Reb proteins (R-body components) in complete bacterial genomes. We found that reb genes are much more widespread than previously thought, being present in representatives of major Proteobacterial subdivisions, including many free-living taxa, as well as taxa known to be involved in various kinds of interactions with eukaryotes, from mutualistic associations to pathogenicity. Reb proteins display very good conservation at the sequence level, suggesting that they may produce functional R-bodies. Phylogenomic analysis indicates that reb genes underwent a complex evolutionary history and allowed the identification of candidates potentially involved in R-body assembly, functioning, regulation, or toxicity. Our results strongly suggest that the ability to produce R-bodies is likely widespread in Proteobacteria. The potential involvement of R-bodies in as yet unexplored interactions with eukaryotes and the consequent ecological implications are discussed.
Liste complète des métadonnées

Littérature citée [38 références]  Voir  Masquer  Télécharger

https://hal.sorbonne-universite.fr/hal-01579027
Contributeur : Gestionnaire Hal-Upmc <>
Soumis le : mercredi 30 août 2017 - 11:47:44
Dernière modification le : mercredi 21 mars 2018 - 18:57:49

Fichier

505.full.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Kasie Raymann, Louis-Marie Bobay, Thomas G. Doak, Michael Lynch, Simonetta Gribaldo. A Genomic Survey of Reb Homologs Suggests Widespread Occurrence of R-Bodies in Proteobacteria. G3, Genetics Society of America, 2013, 3 (3), pp.505-516. 〈10.1534/g3.112.005231〉. 〈hal-01579027〉

Partager

Métriques

Consultations de la notice

103

Téléchargements de fichiers

19