We propose and evaluate a new type of optical force microscope based on a standing wave optical trap. Our microscope, calibrated in-situ and operating in a dynamic mode, is able to trap, without heating, a single metallic nanoparticle of 150 nm that acts as a highly sensitive probe for external radiation pressure. An Allan deviation-based stability analysis of the setup yields an optimal 0.1 Hz measurement bandwidth over which the microscope is thermally limited. Over this bandwidth, and with a genuine sine-wave external drive, we demonstrate an optical force resolution down to 3 fN in water at room temperature with a dynamical range for force detection that covers almost 2 orders of magnitude. This resolution is reached both in the confined and freely diffusing regimes of the optical trap. In the latter, we measure 10−11 m induced displacements on the trapped nanoparticle, spatially confined within less than 25 nm along the optical axis.